Welcome to the Neurophysiology Lab

 

 

Superimposed brightfield and fluorescence images of a neuron in a brainstem slice preparation.  The recording pipette can be seen on the left, and the arrow points to a secondary pipette used to apply a pharmacologic agent to an apical dendritic process.  Scale bar is 20 microns.

 

Fluorescence image of the calcium influx into a neuron (upper right) during a series of action potentials evoked by depolarizing current pulses (lower right).  The color scale indicates a fluorescence change from 0% (violet) to 30% (red), and the scale bar is 20 microns.  The horizontal scale on traces is 10 ms, the vertical scale is 25 mV and 2.5 nA.